黄地老虎成虫对15种植物挥发物的电生理和行为反应

【目的】 筛选对黄地老虎Agrotis segetum雌性成虫具有引诱活性的植物挥发物,为黄地老虎食诱剂的研发提供候选物质【方法】 利用触角电位仪(EAG)测试黄地老虎雌性成虫对15种植物挥发物的电生理反应,通过风洞试验,评价每种物质对黄地老虎雌性成虫的吸引作用。【结果】 黄地老虎雌性成虫触角对4种挥发物(癸烷、对乙基苯乙酮、2-甲基壬烷和3,3-二甲基辛烷)没有明显的EAG反应,对其余11种挥发物(1,2-二乙苯、1,4-二乙苯、丁酸丁酯、4,8-二甲基-1,3,7-壬三烯、桉叶油醇、乙酸叶醇酯、芳樟醇、β-月桂烯、壬醛、罗勒烯和β-蒎烯)的电生理反应明显,且反应程度随着挥发物浓度的增加而增加,在最大测试浓度100 μg/mL时产生最强烈的电生理反应。在风洞测试中,黄地老虎雌性成虫对1,2-二乙苯、1,4-二乙苯、丁酸丁酯、癸烷、4,8-二甲基-1,3,7-壬三烯、对乙基苯乙酮、桉叶油醇、乙酸叶醇酯、芳樟醇、β-月桂烯、壬醛、3,3-二甲基辛烷、罗勒烯和β-蒎烯14种挥发物表现出明显的行为趋好。【结论】 筛选出对黄地老虎雌性成虫具有电生理和行为吸引活性的植物挥发物组分。     

新疆南疆机采棉品种株型结构与产量性状的相关性

【目的】分析不同类型陆地棉品种的株型结构及产量性状,研究高产优质机采棉品种的株型结构与产量结构的特点,为棉花结构育种提供理论参考。【方法】测试不同机采棉品种的株型结构性状及产量结构相关指标,利用差异显著性分析、相关分析等统计方法,研究几种机采棉品种的株型性状、产量结构性状及其相关性特点。【结果】籽棉产量与株高、主茎节间长、果枝数、果节数存在正相关但不显著,与果枝始节高度呈负相关;株高与果枝始节高存在显著正相关,与主茎节间长度呈极显著相关; 17N11株型为塔型结构,其底部主茎叶片保留完整、籽棉产量、株高最高,而17N13主茎叶片数最多,17N5则具有最小的叶片脱落率;在棉花株型结构的共性方面,棉花果枝角度具有由上至下呈增大趋势,而变异能力则呈现与之相反的趋势(上部＞中部＞下部)。棉花主茎叶大小具有由上部至下部减小的趋势,变异能力亦呈现与之相反的趋势(下部＞中部＞上部)。【结论】株型结构具备中、上部果枝夹角较小(上部＜32.0°,中部＜50.0°),主茎叶总面积高于320 cm²,下部主茎叶片脱落率低于20%的机采棉品种产量优势显著,中上部果枝夹角、主茎叶总面积、下部主茎叶片脱落率可作为株型育种后代材料选择依据。     

Allometric Estimates of Aboveground Biomass Using Cover and Height Are Improved by Increasing Specificity of Plant Functional Groups in Eastern Australian Rangelands

Plant aboveground biomass (AGB) is a useful metric to assess ecosystem functioning, and its sensitivity to changing environmental conditions provides insight into potential global change impacts. Allometric estimates of AGB using vegetation characteristics such as plant cover or height provide nondestructive biomass proxies for repeated measurements but can introduce uncertainty to estimates. We estimated the relationship between both plant cover and a cover·height index and AGB for 15 plant species from six sites to identify the most reliable approach to estimate biomass nondestructively in semiarid eastern Australian rangelands. Estimates were made by grouping species at four different levels of specificity, to test whether generic estimates were more robust than grouping species based on life history and morphological characteristics. Estimates were then tested on a 1.5-m² plot at each site for validation. In all cases, models were highly significant (P < 0.001) with adjusted R² values ranging from 0.42 to 0.96 for cover models and 0.38 to 0.98 for cover·height index models. We found the addition of height improved model fits in four groups while reducing model fits in two groups. The error around AGB estimates for cover·height index−based models ranged from −66.8 to 4% (absolute mean 35%). Cover-based models had errors between −13.4% and 53% (absolute mean 14.2%). For cover-based estimates of AGB in validation plots, grouping plants by plant functional types (PFTs) increased accuracy (absolute mean error 17.3%) compared with estimates using data from all 15 species (absolute mean of 65.2%). Overall cover was a useful surrogate to estimate AGB (with the exception of one site, accuracy ranged from −2.3% to 11.5%), while height (thought to be a surrogate for canopy characteristics) provided benefit in a few circumstances. We suggest that future research should test additional nondestructive proxies and group species based on PFTs to improve AGB estimates using allometry.     

濒危植物太行花的组织培养方法

以濒危植物太行花顶芽为试材,采用植物组织培养方法,研究了植物生长调节剂对太行花无菌苗丛生芽诱导和生根的影响,以期为太行花快速繁殖、资源保护和园林应用提供参考依据。结果表明:在MS+6-BA 0.6 mg·L^-1+IBA 0.15 mg·L^-1培养基中,太行花丛生芽增殖系数较高且无玻璃化现象,为优化的增殖培养基;在MS+IBA 1.5 mg·L^-1培养基中太行花无菌苗生根率为100%,生根系数也最高,可达4.96,为优化的生根培养基。     

基于几何模型的绿萝叶片外部表型参数三维估测

为快速高效获取叶类植物叶片的外部表型参数、掌握植株生长状况，以绿萝叶片为研究对象，提出一种基于几何模型的叶长、叶宽与叶面积的三维估测方法。利用微软Kinect V2相机，自80cm高度垂直位姿获取绿萝叶片局部点云，并进行直通滤波去噪与包围盒精简等预处理，测量得到点云外形参数，输入预先建立的SAE网络分类预测得到几何模型参数，并基于曲面参数方程建立叶片几何模型。采用粒子群优化算法计算几何模型离散点云和局部点云间的空间距离，进行空间匹配，利用遗传算法求解最优匹配模型的内部模型参数，输出最优匹配模型的叶长、叶宽与叶面积作为估测结果。实验共采集150片绿萝叶片的局部点云数据，将估测结果和真实值进行数学统计与线性回归分析，得出叶长、叶宽与叶面积估测的平均误差分别为0.46cm、0.41cm和3.42cm2，叶长估测R2和RMSE分别为0.88和0.52cm，叶宽R2和RMSE分别为0.88和0.52cm，叶面积R2和RMSE分别为0.95和3.60cm2。实验表明，该方法对于绿萝叶片外形参数的估测效果较好，具有较高实用价值。     

大豆植株分杈数自动提取算法研究

根据大豆植株分杈个数可以预估大豆的产量。为了满足现代农业预测农产品产量的需要,本文提出一种大豆植株分杈数自动提取算法,即首先将采集到的大豆植株图片进行灰度变换、滤波、阈值分割得到二值图像,再进行形态学中的闭运算操作得到大豆植株的轮廓,然后基于Zhang并行快速细化算法提取骨架,最后借助改进的角点检测算法对大豆植株的所有分杈点进行标记并自动统计分杈数,仿真结果与大豆植株实际分杈点一致。     

不同发育阶段马尾松人工林经营效果动态分析

为揭示不同发育阶段马尾松人工林经营效果的动态变化特征规律,对青羊湖林场不同发育阶段的马尾松人工林(幼龄林、中龄林、近熟林)林分空间结构、物种多样性、土壤肥力、林木生长等指标进行动态分析,运用主成分分析法,对不同发育阶段的各经营样地的经营效果进行综合评价。结果表明:不同发育阶段的马尾松林在经营改造后,空间结构明显改善,物种多样性指数均明显提高,土壤肥力也有所改善;幼龄林、中龄林和近熟林9块样地抚育间伐补植改造效果综合评价得分依次为0.079,-0.021,-0.241,0.528,0.438,1.028,-0.145,-0.780,-0.886,得分越高说明改造经营效果越好。经营促进了不同发育阶段马尾松人工林林分空间结构的优化、树种多样性的提高、土壤肥力的改善和林木蓄积生长的提高,林分向异龄复层混交林方向发展,为马尾松人工林经营提供理论依据。     

氨基酸钙对烤烟生长及烟叶品质的影响

为了进一步优化烤烟营养调控技术、提升烟叶品质,采取大田试验的方式,研究了氨基酸钙对烤烟生长以及内在品质的影响。结果表明,施用氨基酸钙后烤烟田间长势较好,平顶期烟株株高、叶面积等农艺性状指标均优于常规对照,烤后烟叶具有较高的均价和上中等烟比例,能够增加烤后烟叶的经济效益。施用氨基酸钙对烟叶品质具有一定的改善作用,可以提升烤后上部烟叶的两糖含量,并对烟碱含量具有一定的降低作用,在团棵期和旺长期分次施用氨基酸钙B2F和C3F等级烟叶的感官评吸质量最好。综合分析,在本试验条件下,氨基酸钙的施用方式以在团棵期或旺长期分次施用较为适宜。     

芝麻不同生育阶段需水特性与干旱灌溉应用研究

探讨芝麻不同生育阶段需水特性与干旱灌溉水分生产效率,为芝麻高产栽培的合理灌溉和水分管理提供科学依据,2013-2018年在安徽、新疆等不同地域,选用6个芝麻主栽品种,开展芝麻不同生育阶段需水特性和灌溉水利用研究。结果表明：盆栽芝麻的全生育期蒸腾需水量平均为283.59mm,品种间差异大,形成100kg芝麻籽粒蒸腾需水量为263.56mm(175.7t/666.7m²),苗期、蕾期、初花期、盛花期、终花期、成熟期的蒸腾需水量分别为18.76mm、21.91mm、21.03mm、149.28mm、71.21mm和34.79mm,蒸腾模系数为5.89%、7.21%、6.85%、47.07%、23.93%和9.05%；盛花期最大,出苗期最小。池栽芝麻全生育期需水量531.36mm,其中株间蒸发量、蒸腾量分别占全生育期需水量的46.1%和53.9%；株间蒸发量最大的时期为苗期；蒸腾量最大的时期为花期。芝麻苗期、花期和成熟期需水模系数平均为18.65%、66.79%和14.56%。合肥基地出苗期、临泉基地花期遇旱喷灌,水分生产效率高达0.61kg/m³和0.65kg/m³；新疆精河基地按需滴灌处理比传统滴灌方式的水分生产效率提高43.28%,节水19.61%,这对水资源匮乏的新疆干旱区来说意义重大。芝麻需水量与栽培条件、品种、气温(r＝0.99)等密切相关。在芝麻不同生育阶段遇旱灌溉是提高水分生产效率和产量的关键措施之一。     

An efficient method to produce segregating populations in quinoa (Chenopodium quinoa)

Quinoa offers a promising alternative for staple food, considering its outstanding nutritional value and tolerance to abiotic stresses. To develop breeding programmes in quinoa, a reliable crossing method for increasing the genetic variation is required. In the following study, we aimed to develop segregating populations in quinoa. We tested the efficiency of three different crossing methods (hand emasculation, warm water emasculation and no emasculation). Moreover we developed a two-stage selection strategy based on morphological traits and molecular markers for the selection of hybrid plants. We reported hand emasculation to be the most efficient crossing method, followed by warm water emasculation and no emasculation. Our results demonstrated that crosses in quinoa can be successfully performed, despite its complicated flower structure and high self-pollination rate. Additionally, we developed 30 segregating populations from crosses between accessions of different origins with varying phylogenetic relationship, which offers a promising perspective for quinoa breeding programmes in the future.